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rrid ab 2616025 anti dnmt1 cell signaling  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rrid ab 2616025 anti dnmt1 cell signaling
    Rrid Ab 2616025 Anti Dnmt1 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dnmt1/pm41875887-824-88-91
    Average 86 stars, based on 1 article reviews
    rrid ab 2616025 anti dnmt1 cell signaling - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: Molecular profiling of chemotherapy-resistant breast cancer reveals DNA methylation remodeling associated with the acquisition of paclitaxel resistance.
    Article Snippet: The membranes were subsequently blocked for 1 h in 5 % non-fat milk (PanReac Applichem, Germany) diluted in Tris-buffered saline (TBS). .. The membranes were incubated with DNMT1 (cat. no. 5032; Cell Signaling Technology, USA), DNMT3A (cat. no. 3598; Cell Signaling Technology, USA), DNMT3B (cat. no. 67259; Cell Signaling Technology, USA), TET2 (cat. no. 18950; Cell Signaling Technology, USA), TET3 (cat. no. 85016; Cell Signaling Technology, USA), CDA (cat. no. PA5–84630; Invitrogen, USA), DHFR (cat. no. 43497; Cell Signaling Technology, USA) or DCK (cat. no. PA5–27787; Invitrogen, USA) primary antibodies at 4◦C overnight, followed by incubation with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 680 (Invitrogen, USA) for 1 h at room temperature (RT). .. Protein stains were detected using the Odyssey® Fc imaging system (LICOR Biotechnology, USA) at 700 nm.

    Article Title: DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312.
    Article Snippet: .. After blocking with 5% non-fat milk, membranes were incubated overnight at 4°C with primary antibodies (all diluted at 1:1000) against DUSP26 (Invitrogen, PA5-22013), p53 (Cell Signaling Technology, 2524S), p-p53 (S15) (Cell Signaling Technology, 9284S), p-p53 (S20) (Cell Signaling Technology, 9287T), p-p53 (S46) (Proteintech, 28960), cleaved caspase-3 (Cell Signaling Technology, 9664S), NGAL (R&D Systems, AF1857), KIM-1 (R&D Systems, AF1817), GAPDH (Proteintech, 10494), β-actin (Proteintech, 66009), DNMT1 (Cell Signaling Technology, 5032), DNMT3a (abcam, ab188470), DNMT3b (abcam, ab2851) and FLAG (Sigma-Aldrich, 102698217), as appropriate. .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (1:5000; Proteintech).

    Ubiquitin Proteomics:

    Article Title: Study on epigenetic regulatory mechanism of MBD4 with a germline loss-of-function mutation (rs140693) responsible for retinoblastoma chemoresistance via the cell cycle and apoptotic pathways.
    Article Snippet: .. The primary antibodies used included β-actin, MBD4, ubiquitin, RB1 (Abcam, Cambridge, UK), MSH2, MSH6, PMS2 (Abways Biotech, Shanghai, China), FADD (Proteintech Group, Rosemont, IL, USA), USP7, Caspase3, cleaved caspase3, Caspase-8, cleaved caspase8, MLH1, DNMT1, HDAC1, P21, P18, P57, FLIP and cleaved PARP (Cell Signaling Technology, Danvers, MA, USA). .. The following reagents were used: carboplatin (Cat. No. C2538, Sigma–Aldrich), etoposide (Cat. NO. E1383, Sigma–Aldrich), cycloheximide (CHX, Cat. NO, 5.08739, Sigma–Aldrich), MG132 (Cat. NO. M8699, Sigma–Aldrich), and bafilomycin A1 (Baf A1, Cat. NO. 196000, Sigma–Aldrich).

    Electrophoresis:

    Article Title: FBXW4 suppresses the proliferation and migration of lung adenocarcinoma cells by inhibiting PKNOX2 promoter methylation.
    Article Snippet: Globally, lung cancer ranks as the second most frequently diagnosed malignancy and the primary driver of cancerrelated deaths (Xia et al. 2022).. Non-small cell lung cancer (NSCLC) is its predominant histopathological subtype (Li et al. 2023), with lung adenocarcinoma (LUAD) being the most frequent subtype (Hernandez-Martinez et al. 2023).. Beyond genetic alterations, chronic lung tissue injury and repair mechanisms induced by environmental exposures play a critical role in the pathogenesis and progression of LUAD (Singh et al. 2025; Kumari et al. 2024a, b; Payal Singh et al. 2023).

    Membrane:

    Article Title: FBXW4 suppresses the proliferation and migration of lung adenocarcinoma cells by inhibiting PKNOX2 promoter methylation.
    Article Snippet: Globally, lung cancer ranks as the second most frequently diagnosed malignancy and the primary driver of cancerrelated deaths (Xia et al. 2022).. Non-small cell lung cancer (NSCLC) is its predominant histopathological subtype (Li et al. 2023), with lung adenocarcinoma (LUAD) being the most frequent subtype (Hernandez-Martinez et al. 2023).. Beyond genetic alterations, chronic lung tissue injury and repair mechanisms induced by environmental exposures play a critical role in the pathogenesis and progression of LUAD (Singh et al. 2025; Kumari et al. 2024a, b; Payal Singh et al. 2023).

    Blocking Assay:

    Article Title: DUSP26 protects against acute kidney injury by dephosphorylating p53 at serine 312.
    Article Snippet: .. After blocking with 5% non-fat milk, membranes were incubated overnight at 4°C with primary antibodies (all diluted at 1:1000) against DUSP26 (Invitrogen, PA5-22013), p53 (Cell Signaling Technology, 2524S), p-p53 (S15) (Cell Signaling Technology, 9284S), p-p53 (S20) (Cell Signaling Technology, 9287T), p-p53 (S46) (Proteintech, 28960), cleaved caspase-3 (Cell Signaling Technology, 9664S), NGAL (R&D Systems, AF1857), KIM-1 (R&D Systems, AF1817), GAPDH (Proteintech, 10494), β-actin (Proteintech, 66009), DNMT1 (Cell Signaling Technology, 5032), DNMT3a (abcam, ab188470), DNMT3b (abcam, ab2851) and FLAG (Sigma-Aldrich, 102698217), as appropriate. .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (1:5000; Proteintech).

    Proximity Ligation Assay:

    Article Title: RAC1 Regulates SHH-Medulloblastoma Growth via GLI-Mediated Transcription.
    Article Snippet: PLA was performed on cells using a Duolink Proximity Ligation Assay (Millipore Sigma) as described by the manufacturer. .. The following antibodies were used for PLA: UHRF1 (catalog no. sc-373750, Santa Cruz Biotechnology, RRID: AB_10947236), DNMT1 (catalog no. 5032, Cell Signaling Technology, RRID: AB_10548197), GLI1 (catalog no. AF3455, R&D Systems, RRID: AB_2247710), GLI2 (catalog no. AF3635, R&D Systems, RRID: AB_2111902), and rabbit IgG (catalog no. 12370, Millipore Sigma). .. DAPI staining was performed using ProLong Gold Antifade Mountant with DAPI (Invitrogen).

    Reverse Transcription:

    Article Title: Loss of MicroRNA-29b promotes DNMT3b-mediated STING downregulation to attenuate radiotherapy-induced antitumor immunity in KRAS-mutated colorectal cancer.
    Article Snippet: .. AR TI CL E IN P RE SS The following antibodies were used: phospho-STING-S365 (#19781, Cell Signaling Technology), STING (#13647, Cell Signaling Technology), cleaved caspase-3 (#9661, Cell Signaling Technology and IR96–401, iReal Biotech.), calreticulin (#12238, Cell Signaling Technology), beta-actin (Ab8227, Abcam), DNMT1 (#5032, Cell Signaling Technology), DNMT3a (#49768, Cell Signaling Technology), DNMT3b (#57868, Cell Signaling Technology), KRAS (A1190, ABclonal) and GAPDH (IR3-8, iReal Biotech.) qRT‒PCR Total RNA was extracted from the cell lines with TRIzol (Invitrogen, CA, USA), quantified by measuring the absorbance at 260 nm, and then reverse-transcribed into cDNA using iScriptTM Reverse Transcription Supermix (Bio-Rad, CA, USA) according to the manufacturer’s instructions (31). .. Primers were designed via the Primer Design Tool (NCBI, USA) according to sequence information from the NCBI database. qRT‒PCR was performed in a final volume of 20 μL with iQTM SYBR® Green Supermix (Bio-Rad, CA, USA) via the CFX96 Touch Real-Time PCR Detection System (Bio-Rad).



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    Image Search Results


    Effects of electroacupuncture (EA) combined with repetitive transcranial magnetic stimulation (rTMS) on motor function and neuroregeneration-related protein expression in the HIBD-induced cerebral palsy rat model. A ) Behavioral assessments of rotarod latency, grid-walking test, and grip strength test across the Control, HIBD Model, EA, rTMS, and EA+rTMS groups. B ) Western blot analysis of protein expression levels for GAP-43, MBP, PI3K, total AKT, phosphorylated AKT (p-AKT), and DNMT1. C ) Quantitative real-time PCR (qRT-PCR) analysis of mRNA expression levels for GAP-43, MBP, PI3K, total AKT, and DNMT1. Data are presented as mean ± SD (n=6 rats per group); *p <0.05, ** *p <0.01, *** *p <0.001.

    Journal: European Journal of Histochemistry : EJH

    Article Title: Electroacupuncture combined with rTMS promotes neuronal regeneration via DNMT1-mediated PI3K-AKT pathway in cerebral palsy model

    doi: 10.4081/ejh.2026.4533

    Figure Lengend Snippet: Effects of electroacupuncture (EA) combined with repetitive transcranial magnetic stimulation (rTMS) on motor function and neuroregeneration-related protein expression in the HIBD-induced cerebral palsy rat model. A ) Behavioral assessments of rotarod latency, grid-walking test, and grip strength test across the Control, HIBD Model, EA, rTMS, and EA+rTMS groups. B ) Western blot analysis of protein expression levels for GAP-43, MBP, PI3K, total AKT, phosphorylated AKT (p-AKT), and DNMT1. C ) Quantitative real-time PCR (qRT-PCR) analysis of mRNA expression levels for GAP-43, MBP, PI3K, total AKT, and DNMT1. Data are presented as mean ± SD (n=6 rats per group); *p <0.05, ** *p <0.01, *** *p <0.001.

    Article Snippet: 3) Hypoxia + DNMT1-KD group: following 3 h of hypoxia, neurons were transfected with DNMT1 siRNA (100 nM total concentration, custom sequence; Sangon Biotech, Shanghai, China) using Lipofectamine 3000 (L3000015; ThermoFisher, Waltham, MA, USA) and Opti-MEM (31985070; ThermoFisher) for 6 h, then cultured for an additional 24 h in Neurobasal-A medium under normoxia.

    Techniques: Expressing, Control, Western Blot, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    Electroacupuncture (EA) combined with repetitive transcranial magnetic stimulation (rTMS) enhances neuronal regeneration and modulates DNMT1, GAP-43, and PI3K expression in the rat spinal cord and cerebral cortex. A ) Nissl staining showing neuronal morphology and density in the cerebral cortex across the Control, HIBD Model, EA, rTMS, and EA+rTMS groups, with the EA+rTMS group demonstrating the greatest improvement in neuronal integrity; magnification: 200×. B ) Immunofluorescence staining of DNMT1, GAP-43, and PI3K (with DAPI nuclear counterstain) in the spinal cord and cerebral cortex across all experimental groups; magnification: 200×. C ) Quantitative analysis of relative fluorescence intensity for DNMT1, GAP-43, and PI3K from the immunofluorescence staining assays. Data are presented as mean ±SD (n=6 rats per group); * p <0.05, ** p <0.01, *** p <0.001.

    Journal: European Journal of Histochemistry : EJH

    Article Title: Electroacupuncture combined with rTMS promotes neuronal regeneration via DNMT1-mediated PI3K-AKT pathway in cerebral palsy model

    doi: 10.4081/ejh.2026.4533

    Figure Lengend Snippet: Electroacupuncture (EA) combined with repetitive transcranial magnetic stimulation (rTMS) enhances neuronal regeneration and modulates DNMT1, GAP-43, and PI3K expression in the rat spinal cord and cerebral cortex. A ) Nissl staining showing neuronal morphology and density in the cerebral cortex across the Control, HIBD Model, EA, rTMS, and EA+rTMS groups, with the EA+rTMS group demonstrating the greatest improvement in neuronal integrity; magnification: 200×. B ) Immunofluorescence staining of DNMT1, GAP-43, and PI3K (with DAPI nuclear counterstain) in the spinal cord and cerebral cortex across all experimental groups; magnification: 200×. C ) Quantitative analysis of relative fluorescence intensity for DNMT1, GAP-43, and PI3K from the immunofluorescence staining assays. Data are presented as mean ±SD (n=6 rats per group); * p <0.05, ** p <0.01, *** p <0.001.

    Article Snippet: 3) Hypoxia + DNMT1-KD group: following 3 h of hypoxia, neurons were transfected with DNMT1 siRNA (100 nM total concentration, custom sequence; Sangon Biotech, Shanghai, China) using Lipofectamine 3000 (L3000015; ThermoFisher, Waltham, MA, USA) and Opti-MEM (31985070; ThermoFisher) for 6 h, then cultured for an additional 24 h in Neurobasal-A medium under normoxia.

    Techniques: Expressing, Staining, Control, Immunofluorescence, Fluorescence

    DNMT1 knockdown enhances PI3K-AKT signaling and promotes axonal regeneration in hypoxia-exposed primary rat cortical neurons. A ) Western blot analysis of protein expression levels for DNMT1, PI3K, total AKT, phosphorylated AKT (p-AKT), and GAP-43 across the Control, Hypoxia, Hypoxia+DNMT1-KD, Hypoxia+DNMT1-OE, Hypoxia+PI3K Inhibition, and Hypoxia+DNMT1-KD+PI3K-OE groups, with corresponding quantitative analyses below. B ) Quantitative real-time PCR (qRT-PCR) analysis of mRNA expression levels for DNMT1, PI3K, GAP-43, and total AKT in each experimental group. Data are presented as mean ±SD (n=6 independent replicates per group); * p <0.05, ** p <0.01, *** p <0.001.

    Journal: European Journal of Histochemistry : EJH

    Article Title: Electroacupuncture combined with rTMS promotes neuronal regeneration via DNMT1-mediated PI3K-AKT pathway in cerebral palsy model

    doi: 10.4081/ejh.2026.4533

    Figure Lengend Snippet: DNMT1 knockdown enhances PI3K-AKT signaling and promotes axonal regeneration in hypoxia-exposed primary rat cortical neurons. A ) Western blot analysis of protein expression levels for DNMT1, PI3K, total AKT, phosphorylated AKT (p-AKT), and GAP-43 across the Control, Hypoxia, Hypoxia+DNMT1-KD, Hypoxia+DNMT1-OE, Hypoxia+PI3K Inhibition, and Hypoxia+DNMT1-KD+PI3K-OE groups, with corresponding quantitative analyses below. B ) Quantitative real-time PCR (qRT-PCR) analysis of mRNA expression levels for DNMT1, PI3K, GAP-43, and total AKT in each experimental group. Data are presented as mean ±SD (n=6 independent replicates per group); * p <0.05, ** p <0.01, *** p <0.001.

    Article Snippet: 3) Hypoxia + DNMT1-KD group: following 3 h of hypoxia, neurons were transfected with DNMT1 siRNA (100 nM total concentration, custom sequence; Sangon Biotech, Shanghai, China) using Lipofectamine 3000 (L3000015; ThermoFisher, Waltham, MA, USA) and Opti-MEM (31985070; ThermoFisher) for 6 h, then cultured for an additional 24 h in Neurobasal-A medium under normoxia.

    Techniques: Knockdown, Western Blot, Expressing, Control, Inhibition, Real-time Polymerase Chain Reaction, Quantitative RT-PCR

    DNMT1 knockdown promotes cell viability and axonal regeneration in hypoxia-exposed primary rat cortical neurons. A ) Cell viability detected by CCK-8 assay across the Control, Hypoxia, Hypoxia+DNMT1-KD, Hypoxia+DNMT1-OE, Hypoxia+PI3K Inhibition, and Hypoxia+DNMT1-KD+PI3K-OE groups. B ) Immunofluorescence staining showing the distribution and expression of DNMT1 (green) and GAP-43 (red), with DAPI (blue) nuclear counterstain and merged images; quantitative analysis of relative fluorescence intensity for DNMT1 and GAP-43 is shown below; magnification: 200×. C ) Axonal length assessment via SAP102 staining (green) with DAPI (blue) nuclear counterstain, and corresponding quantitative analysis of axonal length; magnification: 200×. Data are presented as mean ±SD (n=6 independent replicates per group); * p <0.05, ** p <0.01, *** p <0.001.

    Journal: European Journal of Histochemistry : EJH

    Article Title: Electroacupuncture combined with rTMS promotes neuronal regeneration via DNMT1-mediated PI3K-AKT pathway in cerebral palsy model

    doi: 10.4081/ejh.2026.4533

    Figure Lengend Snippet: DNMT1 knockdown promotes cell viability and axonal regeneration in hypoxia-exposed primary rat cortical neurons. A ) Cell viability detected by CCK-8 assay across the Control, Hypoxia, Hypoxia+DNMT1-KD, Hypoxia+DNMT1-OE, Hypoxia+PI3K Inhibition, and Hypoxia+DNMT1-KD+PI3K-OE groups. B ) Immunofluorescence staining showing the distribution and expression of DNMT1 (green) and GAP-43 (red), with DAPI (blue) nuclear counterstain and merged images; quantitative analysis of relative fluorescence intensity for DNMT1 and GAP-43 is shown below; magnification: 200×. C ) Axonal length assessment via SAP102 staining (green) with DAPI (blue) nuclear counterstain, and corresponding quantitative analysis of axonal length; magnification: 200×. Data are presented as mean ±SD (n=6 independent replicates per group); * p <0.05, ** p <0.01, *** p <0.001.

    Article Snippet: 3) Hypoxia + DNMT1-KD group: following 3 h of hypoxia, neurons were transfected with DNMT1 siRNA (100 nM total concentration, custom sequence; Sangon Biotech, Shanghai, China) using Lipofectamine 3000 (L3000015; ThermoFisher, Waltham, MA, USA) and Opti-MEM (31985070; ThermoFisher) for 6 h, then cultured for an additional 24 h in Neurobasal-A medium under normoxia.

    Techniques: Knockdown, CCK-8 Assay, Control, Inhibition, Immunofluorescence, Staining, Expressing, Fluorescence